
Jonathan "Jack" Donahue Bristow, played by Victor Garber, is a fictional character of the ABC television series Alias. He is the father of the central character Sydney Bristow, played by Jennifer Garner.

Sydney Anne Bristow is a fictional character and the primary focus of the television series Alias. She is an American woman with a Russian-Canadian family background who works as a spy for the CIA.

Francine "Francie" Calfo is a fictional character on the television series Alias, portrayed by Merrin Dungey from 2001–03 and again in 2006. Initially the best friend of lead character Sydney Bristow, Francie is murdered by a lookalike assassin named Allison Doren, who assumes her identity.
Irina Derevko is a fictional character on the television series Alias, and a main character during the second season of the series. Irina, played by Lena Olin, is the mother of the central character, Sydney Bristow.

Marcus R. Dixon is a fictional character on the television series, Alias. He has been one of Sydney Bristow's coworkers and the CIA's Director of Joint Task Force. He is portrayed by Carl Lumbly.

Marshall J. Flinkman is a fictional character on the television series, Alias. Flinkman, portrayed by Kevin Weisman, is the tech geek at SD-6 and then later the CIA. When a field agent is sent on a mission, Marshall serves in a function similar to Q from the James Bond films: as the in-house creator and proprietor of a number of gadgets and sophisticated tools used by field agent. UGO.com named him one of the best TV nerds.

Rachel Gibson is a fictional character played by Rachel Nichols in the final season of the spy-fi television series Alias.
Lauren Reed is a fictional character played by Melissa George in the ABC television series Alias. She is Michael Vaughn's wife during the third season of the series.

Renée Rienne is a fictional character in the spy-fi television series Alias. Played by Élodie Bouchez, she was introduced as a new character for the fifth season. Bouchez appears in the opening credit sequence during the first half of the fifth season; beginning with "S.O.S." Bouchez and her character were removed from the opening and listed as a special guest star.

Nadia Santos is a fictional character in the television series Alias, and a main character during the series' fourth season. She is played by Mía Maestro.

Julian Sark is a recurring fictional character portrayed by David Anders on the ABC television series Alias. The series follows Sydney Bristow, an operative working for an organization called SD-6 which she believes is a covert branch of the Central Intelligence Agency. However, she learns it is actually a subsidiary of the Alliance of Twelve, a crime syndicate which seeks to make profits from stolen intelligence and artifacts. Upon learning the truth Sydney offers her services to the CIA as a double agent with the intention of destroying SD-6 from within.

Arvin Sloane is a fictional character played by Ron Rifkin. He was the former director of SD-6 on the television series, Alias. TV Guide included him in their 2013 list of The 60 Nastiest Villains of All Time.

William "Will" D. Tippin is a fictional character, portrayed by Bradley Cooper. He is one of Sydney Bristow's friends on the ABC television series Alias.

Michael C. Vaughn is a fictional character on the television series Alias. Played by Michael Vartan, Vaughn is one of Sydney Bristow's co-workers and her ongoing love interest. He, like Sydney, is skilled in a variety of areas. His language abilities, shown in various episodes, include French, Spanish, Italian, and others.

Eric Weiss is a fictional character, played by Greg Grunberg, from the television series Alias. He is one of Sydney Bristow's CIA co-workers.