
The Battle Android Trooper (B.A.T.) is a fictional robot from the G.I. Joe: A Real American Hero toyline, comic books and animated series. Created by the evil Cobra Organization, the B.A.Ts provide disposable brute strength to the Cobra army.

Baroness is a fictional character from the G.I. Joe: A Real American Hero toyline by Hasbro, originally appearing in the first issue of the G.I. Joe: A Real American Hero comic series by Marvel Comics in June, 1982. The Baroness is a villainess, associated with the G.I. Joe Team's nemesis Cobra.

Beach Head is a fictional character from the G.I. Joe: A Real American Hero toyline, comic books and animated series. He serves as the G.I. Joe Team's sergeant major and debuted in 1986.

Billy Kessler is a fictional character in the comic book series G.I. Joe: A Real American Hero. He is the son of the main villain of the series, Cobra Commander, but fights against his father and the forces of Cobra Command, learning martial arts under the tutelage of Storm Shadow, who helps him after a botched attempt on Cobra Commander's life. While still a boy, he loses an eye and a leg when a Cobra operative destroys a car in which he is riding, and after recovering he goes on to become a member of his father's greatest enemy, the G.I. Joe team.

Blowtorch is a fictional character from the G.I. Joe: A Real American Hero toyline, comic books and animated series. He is the G.I. Joe Team's original Flamethrower and debuted in 1984.

Major Bludd is a fictional character from the G.I. Joe: A Real American Hero toyline, comic books, and animated series. He is a mercenary working for the Cobra Organization.

Cobra Commander is a fictional character and the main antagonist of the G.I. Joe: A Real American Hero franchise. He is the supreme leader of the terrorist organization Cobra and the archenemy of the G.I. Joe Team. He was portrayed by Joseph Gordon-Levitt in the film G.I. Joe: The Rise of Cobra, and by Luke Bracey with the voice of Robert Baker in the 2013 sequel G.I. Joe: Retaliation.

The Cobra Troopers serve as the basic foot soldiers of the Cobra Organization, as part of the G.I. Joe: A Real American Hero toyline, comic books and animated series. They are generally depicted as legions of uniformed soldiers, nearly all of them masked to appear anonymous, and widely diversified according to specialties and functions. "Viper" is the code-name given to a large majority of the Cobra Troopers. With the exception of the Viper infantry trooper, the code name "Viper" is usually preceded by their area of expertise.

Copperhead is a fictional character from the G.I. Joe: A Real American Hero toyline, comic books and animated series. He is Cobra's Water Moccasin pilot and debuted in 1984.

Cover Girl is a fictional character from the G.I. Joe: A Real American Hero toyline, comic books and animated series. She is a tank jockey for the G.I. Joe Team, specializing in the Wolverine missile tank, and debuted in 1983 on the same team.

Laird James McCullen Destro XXIV, usually referred to simply as Destro, is a fictional character from the G.I. Joe: A Real American Hero toyline, comic books, and cartoon series. He is the leader of the Iron Grenadiers, and founder of M.A.R.S. Industries, a weapons manufacturer and supplier for Cobra. Destro is portrayed by Christopher Eccleston in the 2009 live-action film G.I. Joe: The Rise of Cobra.
Duke is a fictional character from the G.I. Joe: A Real American Hero toyline, comic books, and animated series. He is the G.I. Joe Team's First Sergeant, and debuted in 1983. The character is also featured in both the G.I. Joe: Sigma 6 animated series and comic books. Channing Tatum portrays Duke in the 2009 live-action film, G.I. Joe: The Rise of Cobra, and the 2013 sequel G.I. Joe: Retaliation.

Firefly is a fictional character from the G.I. Joe: A Real American Hero toyline, comic books and animated series. He is a mercenary who works for the Cobra Organization as a saboteur. He is portrayed by Ray Stevenson in the 2013 film G.I. Joe: Retaliation.

Gnawgahyde is a fictional character from the G.I. Joe: A Real American Hero toyline, comic books and animated series of the 1980s. He is affiliated with Cobra as the Dreadnoks' poacher and debuted in 1989.

Grunt is a fictional character from the G.I. Joe: A Real American Hero toyline, comic books and animated series. He is an infantryman with the G.I. Joe Team and debuted in 1982.

Jinx is a fictional character from the G.I. Joe: A Real American Hero toyline, comic books and animated series. She debuted in 1987 as the G.I. Joe Team's female ninja character, and since then her code name has been the identity of several other incarnations of the same character, including one of Snake Eyes' apprentices in G.I. Joe: Sigma 6, Chuckles' undercover contact in G.I. Joe: Cobra, and Storm Shadow's cousin in G.I. Joe: Renegades. She is portrayed by Élodie Yung in the 2013 film G.I. Joe: Retaliation.

Leatherneck is a fictional character from the G.I. Joe: A Real American Hero toyline, comic books and animated series. He is a Marine with the G.I. Joe Team and debuted in 1986.

Metal-Head is a fictional character from the G.I. Joe: A Real American Hero toyline, comic books and animated series. He is a member of the Iron Grenadiers, and first appeared in the 1990 edition of the toyline. He is not to be mistaken for another character with a similar name Metalhead from G.I. Joe Extreme.

Dr. Mindbender is a fictional character from the G.I. Joe: A Real American Hero toyline, comic books and animated series. He is a scientist who works for the fictional terrorist organization Cobra; like other characters in the fictional universe, his characterization has fluctuated from that of comical villain to dangerous fascist depending on the release. He is often allied with the Cobra Commander and Destro. In his first live-action film appearance in 2009, he was played by American actor Kevin J. O'Connor.

The Night Creepers are fictional characters from the G.I. Joe: A Real American Hero toyline, comic books and animated series. They are a syndicate of hi-tech ninjas/corporate mercenaries hired by Cobra as spies and assassins.

Overkill is a character from the G.I. Joe: A Real American Hero toyline from Hasbro, which has spawned comics and animated series. He first appeared in the 1989 G.I. Joe: A Real American Hero incarnation of the series. He also appears in G.I. Joe: Sigma 6.

Quick Kick is a fictional character from the G.I. Joe: A Real American Hero toyline, comic books and animated series. He is the G.I. Joe Team's martial arts expert, and debuted in 1985.

Scrap-Iron is a fictional character from the G.I. Joe: A Real American Hero toyline, comic books and animated series. He is Cobra's anti-armor specialist and debuted in 1984.

Serpentor is a fictional character and a recurring antagonist from the G.I. Joe: A Real American Hero toyline, comic books, and animated series. Introduced in 1986, the character rules as Emperor over Cobra. Serpentor serves as the primary antagonist of the second season of the original animated series.

Slice is a fictional character from the G.I. Joe: A Real American Hero toyline, comic books and animated series. He is affiliated with Cobra as a ninja swordsman, and debuted in 1992.

Storm Shadow is a fictional character from the G.I. Joe: A Real American Hero toyline, comic books and animated series. He is best known as the Cobra Commander's ninja bodyguard, and for his history with fellow ninja Snake Eyes. Throughout their history, he has changed sides several times; conflicted in loyalties between Cobra, G.I. Joe, and his blood brother, Snake Eyes. Storm Shadow is one of the most prominent characters in the G.I. Joe: A Real American Hero franchise, and has appeared in every series since its inception. He is portrayed by Lee Byung-hun in the 2009 live-action film G.I. Joe: The Rise of Cobra, and the 2013 sequel G.I. Joe: Retaliation. Andrew Koji portrayed Storm Shadow in the 2021 film, Snake Eyes.

Tomax and Xamot Paoli are fictional characters from the G.I. Joe: A Real American Hero toyline, comic books and animated series. They are the co-leaders of Cobra's elite troops, the Crimson Guard, and debuted in 1985. Their preliminary names were Movat and Tovam respectively.

Torch is a fictional character from the G.I. Joe: A Real American Hero toyline, comic books and animated series. He is affiliated with Cobra as one of the Dreadnoks and debuted in 1985. He is often seen working closely with Ripper and Buzzer.

Dr. Venom is a fictional character who appears in the Marvel G.I. Joe comic series. He is a scientist who is the inventor of the Brainwave Scanner.

Zandar is a fictional character from the G.I. Joe: A Real American Hero toyline, comic books and animated series. He is affiliated with Cobra as a member of the Dreadnoks. He is portrayed by Matt Gerald in the 2013 film G.I. Joe: Retaliation.
Zarana is a fictional character from the G.I. Joe: A Real American Hero toyline, comic books and animated series. She is affiliated with Cobra as a member of the Dreadnoks.