
Ad-Libs is an improvisational comedy troupe based in Dallas, Texas. Combining improvisation along with video segments, Ad-Libs was conceived by Phil Larsson in 1986. Ad-Libs performs weekly shows at its 200-seat downtown Dallas cabaret style theater and tours across the country for corporate events and performing arts centers.

Asperger's Are Us is an American comedy troupe. They are the first comedy troupe consisting entirely of people with Asperger syndrome, though their shows do not reference autism at all.

Barats and Bereta is the web-based sketch comedy duo of Luke Pierre Barats and Joseph Daniel Bereta. Formed in 2003, the duo originally made videos to amuse friends, often combining real and fictional acts. They met when both were students of Gonzaga University. These videos eventually made it onto their website and onto YouTube. Their viral videos have also earned them the description of an Internet phenomenon. They currently have over 395,000 subscribers on YouTube. Views of videos released in 2006 have topped over ten million and more recent videos have an average of 200,000 views. Both have portrayed characters on the popular YouTube web series The Annoying Orange.

The Compass Players was an improvisational cabaret revue active from 1955 to 1958 in Chicago and St. Louis. Several members went on to form The Second City Theater in 1959.

Dynamite Kablammo is a sketch comedy group based in the San Fernando Valley, established in 2006 by Greg Kaczynski, Matt DeNoto, and Candida Rodriguez. Dana DeRuyck, Dane Biren and Meredith Rensa are also members. DeNoto and Kaczynski write the shows with DeRuyck occasionally contributing.

Hot Thespian Action is a sketch comedy troupe based out of Winnipeg, Manitoba, Canada. They have been nominated three times for "Best Sketch Troupe" at the Canadian Comedy Awards. The group consists of Shannon Guile, Jacqueline Loewen, Garth Merkeley, Ryan Miller, and Jane Testar. They appear yearly at the Winnipeg Fringe Theatre Festival, the Winnipeg Comedy Festival, and many other sketch comedy festivals across North America.

The Janoskians are a YouTube comedy group from Melbourne, Australia, The group consists of elder brother Beau Brooks, twins Luke and Jai Brooks, and friends Daniel Sahyounie and James Yammouni. Yammouni left in 2016.

M.I.'s Westside Comedy Theater is a comedy club, theater, bar, and comedy training center located in Santa Monica, California.

Mega64 is a comedy webseries centered on video games created by Rocco Botte, Derrick Acosta, and Shawn Chatfield, formed in 2003. Originally conceived to be a public-access television show, the original five episodes produced were later released on DVD as Mega64: Version 1 in 2004.

Mixed Company of Yale is an all-gender undergraduate a cappella group from Yale University. Founded in 1981, Mixed Company was originally formed as a group specializing in both sketch comedy and a cappella. Today, the group focuses primarily on singing, though the group often integrates skits and other comedy into performances. The group has toured on five continents and regularly performs for world leaders visiting Yale's campus. Mixed Company has released 17 studio albums, and their most recent recording, Animal Flow, was released in summer 2019.

Olde English was an American sketch comedy troupe based in New York City. The group published over 100 comedy shorts on the Internet, and performed live at several venues and festivals in the US.
Oui Be Negroes is an African-American improvisational sketch comedy ensemble, founded by Artistic Director Shaun Landry and Director Hans Summers. They worked together for years in Chicago with the Underground Theatre Conspiracy, and had thrown around the idea of an African-American-driven sketch and improvisational comedy troupe, which would be geared specifically toward social and political humor.

Second Nature is a long-form improvisational theatre troupe based in Los Angeles at the University of Southern California.

SF Sketchfest, or The San Francisco Comedy Festival, is an American comedy festival founded in 2002 by Bay Area actor-comedians David Owen, Janet Varney and Cole Stratton. It takes place every January and February in a variety of Bay Area venues and features sketch comedy, improv comedy, film and television tributes and reunions, solo performers, music, podcasts and stand-up comedy performers.

The Sketchersons are a sketch comedy troupe based in Toronto, Ontario. They are winners of the 2007 Canadian Comedy Award for best Sketch Troupe, an award they had been nominated for each of the 3 years prior. The Sketchersons were again nominated for the award in 2008, 2009, 2010, and 2013.

The Super Serious Show is a live comedy show that mixes stand-up, sketch, musical comedy and videos from a blend of up-and-coming performers and established comedians. Embracing a wide variety of non-polished acts and spontaneity, The Super Serious Show combines elements of tradition club comedy and alternative comedy. The show is often a platform for well-known comics to test new material and "riff," or perform without any planned content. The monthly Los Angeles event features a D.J., food trucks, free food and wine, along with surprise appearances from special guests. In addition to the L.A. installment, the show tours the comedy festival circuit and has appeared at the Edinburgh Festival Fringe, Riot L.A., and South by Southwest in conjunction with Funny or Die and SoundCloud. Notable past performers include Daniel Tosh, Sarah Silverman, "Weird Al" Yankovic, Amy Schumer, Aisha Tyler, Aziz Ansari, Chris Kattan, T.J. Miller, Reggie Watts, Margaret Cho, Demetri Martin, Kristen Schaal, and John Hodgman.

Under the Gun Theater is a theater company located in Chicago, Illinois. Founded by Angie McMahon and Kevin Mullaney, Under the Gun is a sketch and improvisational comedy theater which opened in Chicago's Lake View community in 2014. The theater was known for its interactive show Comedy Against Humanity, which ended due to legal concerns, based on the game Cards Against Humanity. In September 2017 Under the Gun Theater announced it would partner with the Chicago stand-up comedy institution Lincoln Lodge to focus on producing stand-up comedy shows.

The Valleyfolk is a YouTube-based comedy group originating in Los Angeles, California. The group was founded by former SourceFed hosts Joe Bereta, Elliott Morgan, Lee Newton, and Steve Zaragoza. The channel launched in January 2018 and gained over 93,000 subscribers within the first 24 hours.