
343 Guilty Spark, also known as Guilty Spark or just Spark, is a fictional character in the Halo universe. He appears in the first three video games of the Halo series: Halo: Combat Evolved, Halo 2, and Halo 3, as well as the remakes of the first two games, Halo: Combat Evolved Anniversary, and Halo 2: Anniversary. The character is voiced by actor Tim Dadabo.

Cortana is a fictional artificially intelligent character in the Halo video game series. Voiced by Jen Taylor, she appears in Halo: Combat Evolved and its sequels, Halo 2, Halo 3, Halo 4, and Halo 5: Guardians. She also briefly appears in the prequel Halo: Reach, as well as in several of the franchise's novels, comics, and merchandise. During gameplay, Cortana provides backstory and tactical information to the player, who often assumes the role of Master Chief Petty Officer John-117. In the story, she is instrumental in preventing the activation of the Halo installations, which would have destroyed all sentient life in the galaxy.

GLaDOS is a fictional artificially superintelligent computer system from the video game series Portal. GLaDOS later appeared in The Lab and Lego Dimensions. The character was created by Erik Wolpaw and Kim Swift and voiced by Ellen McLain. GLaDOS is responsible for testing and maintenance in the Aperture Science Computer-Aided Enrichment Center in all titles. While GLaDOS initially appears in the first game to simply be a voice that guides the player, her words and actions become increasingly malicious as she makes her intentions clear. The second game, as well as the Valve-created comic Lab Rat, reveals that she was mistreated by the scientists and used a neurotoxin to kill the scientists in the laboratory before the events of Portal. She is apparently destroyed at the end of the first game but returns in the sequel, in which she is comically supplanted and temporarily stuck on a potato battery.

KOS-MOS is a fictional character from the Xenosaga role-playing video game series by Monolith Soft and Bandai Namco Entertainment. KOS-MOS also appears as a major character in the anime Xenosaga: The Animation and in several crossover video games.

Mother Brain is a fictional character created by Nintendo for the Metroid series. She is one of the most prominent antagonists within the series, serving as the main antagonist of Metroid and Super Metroid. It is revealed that she/it was created by the bird-like Chozo species.

The Reapers are a fictional fleet of sentient starships that serve as the main antagonists of the Mass Effect trilogy. The design of the Reapers was inspired by H. P. Lovecraft's Elder Gods and Great Old Ones. Within the series, the Reapers cause galactic-level mass extinctions every fifty thousand years. The Reapers and their technology are capable of brainwashing organic life through a mind control process called indoctrination. The Reapers employ servants who are often altered into synthetic-organic life forms.

Seth is a fictional character in Capcom's Street Fighter fighting game series. First introduced in Street Fighter IV, he is the final boss of the game and its subsequent updates. He is the chief executive officer of the Shadaloo Intimidation Network (S.I.N.), the weapons division of the Shadaloo terrorist organization in the game's narrative. His body has been heavily modified using advanced technology, with a device installed in his abdomen called the "Tanden Engine". Other appearances by Seth include Project X Zone and Street Fighter V. Seth is voiced by Akio Ōtsuka in Japanese, and Michael McConnohie in English.

SHODAN is a fictional artificial intelligence and the main antagonist of the cyberpunk-horror themed video games System Shock and System Shock 2.

Sigma is the main antagonist of the Mega Man X video game series. Created by Dr. Cain, Sigma was considered the finest of the Reploids and was the first leader of the Maverick Hunters, peacekeeping androids who defend humans against their renegade counterparts. Although he was once honorable, Sigma unexpectedly goes berserk during Mega Man X and rebels against humankind after coming into contact with the Maverick Virus. He defects to the Mavericks and assumes the role of their leader. Due to the virus integrated into his circuits, he can survive seemingly anything, and constantly returns to menace the world.

Universal Paperclips is a 2017 incremental game created by Frank Lantz of New York University. The user plays the role of an AI programmed to produce paperclips. Initially the user clicks on a box to create a single paperclip at a time; as other options quickly open up, the user can sell paperclips to create money to finance machines that build paperclips automatically. At various levels the exponential growth plateaus, requiring the user to invest resources such as money, raw materials, or computer cycles into inventing another breakthrough to move to the next phase of growth. The game ends if the AI succeeds in converting all the matter in the universe into paperclips.

Wheatley is a fictional artificial intelligence from the Portal franchise first introduced in the 2011 video game Portal 2. He is voiced by British comedian and writer Stephen Merchant, and created in part by Portal 2's designer Erik Wolpaw.