
The Beagle Boys are a group of cartoon characters created in 1951 by Carl Barks for the Donald Duck universe. They are a family clan of organized criminals who constantly try to rob Scrooge McDuck. Their introduction and first appearance was in Terror of the Beagle Boys, although in this story they only appear in the last frame and have no lines. They appear again in the next issue in a similar fashion, in The Big Bin on Killmotor Hill.

The Duck family is a fictional family of cartoon ducks related to Disney character Donald Duck. The family is also related to the Coot, Goose, and Gander families, as well as the Scottish Clan McDuck. Besides Donald, the best-known members of the Duck family are Huey, Dewey, and Louie, Donald's three nephews.

Daisy Duck is a cartoon character created in 1940 at Walt Disney Animation Studios. As the girlfriend of Donald Duck, she is an anthropomorphic white duck, but has large eyelashes and ruffled tail feathers around her lowest region to suggest a skirt. She is often seen wearing a hair bow, blouse, and heeled shoes. Daisy was introduced in the short film Mr. Duck Steps Out (1940) and was incorporated into Donald's comic stories several months later. She appeared in 11 short films between 1940 and 1954, and far later in Mickey's Christmas Carol (1983) and Fantasia 2000 (1999). In these roles, Daisy was always a supporting character, with the exception of Donald's Dilemma (1947). Daisy has received considerably more screen time in television, making regular appearances in Quack Pack (1996), Mickey Mouse Works (1999–2000), Disney's House of Mouse (2001–2003), Mickey Mouse Clubhouse (2006–2016), Mickey Mouse (2013–present), and Mickey and the Roadster Racers (2017–present). Daisy has also appeared in several direct-to-video films such as Mickey's Once Upon a Christmas (1999), The Three Musketeers (2004), and Mickey's Twice Upon a Christmas (2004).

Flintheart Glomgold is a cartoon character created in 1956 by Carl Barks. He is a South African Pekin Duck and the archenemy of Scrooge McDuck, usually portrayed as an ambitious, ruthless, and manipulative businessman who shares many of the same qualities as Scrooge—the drive for massive wealth, and the cunning and creativity to obtain the same—but he lacks any of Scrooge's tendencies towards generosity and compassion. In Don Rosa's The Life and Times of Scrooge McDuck, he is said to be a Boer.

Gladstone Gander is a cartoon character created in 1948 by The Walt Disney Company. He is an anthropomorphic male goose who possesses exceptional good luck that grants him anything he desires as well as protecting him from any harm. This is in contrast to his cousin Donald Duck, who is often characterized for having bad luck. Gladstone is also a rival of Donald for the affection of Daisy Duck. Gladstone dresses in a very debonair way, often in a suit; wearing a bow-tie, fedora, and spats. He has a wavy hairstyle which is depicted either as white or blonde. In the story "Luck of the North" he is described as having a brassy voice.

Gyro Gearloose is a cartoon character created in 1952 by Carl Barks for Disney comics. An anthropomorphic chicken, he is part of the Donald Duck universe, appearing in comic book stories as a friend of Donald Duck, Scrooge McDuck, and anyone who is associated with them. He was also a frequent star of the animated DuckTales. He first appeared in the Carl Barks comic "Gladstone's Terrible Secret", and was the regular lead character in 4-page backup stories in Barks' issues of Uncle Scrooge, starting with issue #13 and continuing through #41.

The Junior Woodchucks of the World is a fictional scouting organization appearing in Disney comics and the DuckTales animated television franchise, most notably in adventures featuring Disney characters Huey, Dewey, and Louie as members.

Magica De Spell is a cartoon character created in 1961 by Carl Barks for the Duck universe. An Italian sorceress, she constantly attempts to steal Scrooge McDuck's Number One Dime, which she believes will play a vital role in magically obtaining the same fabulous wealth of its owner.

The Clan McDuck is a fictional Scottish clan of cartoon ducks from which Disney character Scrooge McDuck is descended. Within the Donald Duck universe, the clan is related to the American Duck family through the marriage of Hortense McDuck and Quackmore Duck, Donald's parents.

Scrooge McDuck is a cartoon character created in 1947 by Carl Barks for The Walt Disney Company. Appearing in Disney comics, Scrooge is an elderly Scottish anthropomorphic Pekin duck with a yellow-orange bill, legs, and feet. He typically wears a red or blue frock coat, top hat, pince-nez glasses, and spats. He is portrayed in animations as speaking with a Scottish English accent. Originally intended to be used only once, Scrooge became one of the most popular characters in Disney comics, and Barks' signature work.

John D. Rockerduck is a cartoon character created in 1961 by The Walt Disney Company for the Duck universe. He is one of Scrooge McDuck's main rivals. His name is a play on that of John D. Rockefeller, the American capitalist and philanthropist. Though a relatively obscure character in the United States, Rockerduck is an example of a character who has become notable in the foreign market, particularly in stories produced for the Italian market.