
The National Zoological Park, commonly known as the National Zoo, is one of the oldest zoos in the United States. It is part of the Smithsonian Institution and does not charge admission. Founded in 1889, its mission is to "provide engaging experiences with animals and create and share knowledge to save wildlife and habitats".

Bei Bei is a male giant panda cub who was born and lived at the National Zoo in Washington, D.C., in the United States. He was part of US-China relations panda diplomacy, and was sent to the People's Republic of China on November 19, 2019 at the age of 4. He is now at the Ya’an Bifengxia Base of the Giant Panda Conservation and Research Center in southwest Sichuan province. He is the brother of Tai Shan, Bao Bao, and Xiao Qi Ji.

Sara Hallager is an American biologist in avian management and husbandry, specializing in the care and conservation of flamingos and kori bustards. She is curator of birds at the Smithsonian National Zoo.

Ling-Ling and Hsing-Hsing were two giant pandas given to the United States as gifts by the government of China following President Richard Nixon's visit in 1972. As a gift, the U.S. government sent China a pair of musk oxen.

The Smithsonian Conservation Biology Institute (SCBI) is a unit of the Smithsonian Institution located on a 3,200-acre (13 km2) campus located just outside the town of Front Royal, Virginia. An extension of the National Zoo in Washington, D.C., the SCBI has played a leading role in the fields of veterinary medicine, reproductive physiology and conservation biology since its founding in 1974.

Smokey Bear is an American campaign and advertising icon of the U.S. Forest Service. In the Wildfire Prevention Campaign, which is the longest-running public service announcement campaign in United States history, the Ad Council, the United States Forest Service (USFS), and the National Association of State Foresters (NASF), in partnership with creative agency FCB, employ Smokey Bear to educate the public about the dangers of unplanned human-caused wildfires. A campaign began in 1944 featuring Smokey and the slogan "Smokey Says – Care Will Prevent 9 out of 10 Forest Fires". His slogan changed to "Remember... Only YOU Can Prevent Forest Fires" in 1947 and was associated with Smokey Bear for more than five decades. In April 2001, the message was officially updated to "Only You Can Prevent Wildfires" in response to a massive outbreak of wildfires in natural areas other than forests, and to clarify that Smokey was promoting the prevention of unplanned outdoor fires, not prescribed burns. Smokey has also had other lines throughout the years, but these have remained his central slogans. According to the Ad Council, 80% of outdoor recreationists correctly identified Smokey Bear's image and 8 in 10 recognized the campaign PSAs.

Tian Tian is a 275-pound male giant panda at the National Zoo in Washington D.C. The panda was born on August 27, 1997, at the China Conservation and Research Center for the Giant Panda at the Wolong National Nature Reserve in Sichuan Province, to Yong Ba (mother) and Pan Pan (father). Tian Tian is the half-brother of the San Diego Zoo's Bai Yun.

Uncle Beazley is a life-size fiberglass statue of a triceratops by Louis Paul Jonas. It is located near Lemur Island in the National Zoological Park in Northwest Washington, D.C.