
This is the list of 350 cities and towns in Tunisia. In the list by governorate capitals are shown in bold.

Turres in Byzacena was a Roman and Vandal era colonia (city) in North Africa.

Ariana is a coastal city in north-eastern Tunisia, part the agglomeration of Tunis, also called "Grand Tunis". It is located at the north of Tunis city center, around 36°51′45″N 10°11′44″E. It is the capital of Ariana Governorate and the country's eighth largest city.

Béja is a city in Tunisia. It is the capital of the Béja Governorate. It is located 105 kilometers (65 mi) from Tunis, between the Medjerdah River and the Mediterranean, against the foothills of the Khroumire, the town of Béja is situated on the sides of Djebel Acheb, facing the greening meadows, its white terraces and red roofs dominated by the imposing ruins of the old Roman fortress.
Bizerte or Bizerta, the classical Hippo, is a town of Bizerte Governorate in Tunisia. It is the northernmost city in Africa, located 65 km (40mil) north of the capital Tunis. It is one of the oldest known settlements in Tunisia, having been founded by settlers from the Phoenician port of Sidon around 1100 BC. It is also known as the last town to remain under French control after the rest of the country won its independence from France. The city had 142,966 inhabitants in 2014.

Bordj Toumi or Borj Toumi is a town in Tunisia located at 36°45'33"N 9°43'3"E It is a railway town on the Medjerda river. The town is near Dor el Gabsi west of Tunis and at n elevation of 52 meters above sea level.

Boughrara is a coastal town in central-eastern Tunisia. It is located at around 33°32′16″N 10°40′34″E. During the Roman occupation of North Africa, Boughrara was known as Gigthis.

El Kef, also known as Le Kef, is a city in northwestern Tunisia. It serves as the capital of the Kef Governorate.
Gabès, also spelled Cabès, Cabes, Kabes, Gabbs and Gaps, is the capital city of the Gabès Governorate in Tunisia. It is located on the coast of the Gulf of Gabès. With a population of 152,921, Gabès is the 6th largest Tunisian city.

Gafsa, originally called Capsa in Latin, is the capital of Gafsa Governorate of Tunisia. It lends its Latin name to the Mesolithic Capsian culture. With a population of 105,264, Gafsa is the 9th-largest Tunisian city.

Kalâa Kebira is a town and commune in the Sousse Governorate, Tunisia. As of 2004 it had a population of 45,990.

Henchir-El-Djemel, is a village of North Africa, near Sakiet Ezzit in the Gouvernorat of Safaqis, in Tunisia.

Jendouba Formerly known as Souk El Arba until 30 April 1966) is a city in north-western Tunisia, and capital of the Jendouba Governorate. It is an important crossroads with many road links to other towns such as El Kef, Tabarka, Ain Draham and Béja. The main economic activity is agriculture. It is close to the famous ancient Roman city of Bullaregia or Bulla Regia, as well as the ancient marble quarry of Chemtou.

Kairouan, also spelled Al Qayrawān or Kairwan, is the capital of the Kairouan Governorate in Tunisia. It is a UNESCO World Heritage site. The city was founded by the Umayyads around 670. In the period of Caliph Mu'awiya, it became an important centre for Sunni Islamic scholarship and Quranic learning, and thus attracting many Muslims from various parts of the world, next only to Mecca and Medina. The holy Mosque of Uqba is situated in the city.

Kasserine is the capital city of the Kasserine Governorate, in west-central Tunisia. It is situated below Jebel ech Chambi, Tunisia's highest mountain. Its population is 114.463 (2020).

Mahdia is a Tunisian coastal city with 62,189 inhabitants, south of Monastir and southeast of Sousse.

Majaz al Bab, also known as Medjez el Bab, or as Membressa under the Roman Empire, is a town in northern Tunisia. It is located at the intersection of roads GP5 and GP6, in the Plaine de la Medjerda.
Meninx is a Tunisian archaeological site located on the southeastern coast of the island of Djerba, near the present city of Henchir El Kantara. It stretches 2 kilometers (1.2 mi) long and 800 meters (2,600 ft) wide, some of which has probably been submerged by the sea. The theater is at 33.688° N, 10.925° E.

Monastir, also called Mistīr, is a city on the central coast of Tunisia, in the Sahel area, some 20 kilometres south of Sousse and 162 kilometres south of Tunis. Traditionally a fishing port, Monastir is now a major tourist resort. Its population is about 93,306. It is the capital of Monastir Governorate.

Oum El Abouab also known as Seressi is a town in Zaghouan Governorate, Tunisia that is located at 36° 10′ 00″ N, 9° 46′ 20″E. It is at an altitude of 2000m.

Sfax is a city in Tunisia, located 270 km (170 mi) southeast of Tunis. The city, founded in AD 849 on the ruins of Roman Taparura, is the capital of the Sfax Governorate, and a Mediterranean port. Sfax has a population of 330,440. The main economic activities of Sfax are industries, agriculture, fishing and trade (import-export). The city is often described as Tunisia's "second city", being the second-most populous city after the capital Tunis.
Sousse or Soussa is a city in Tunisia, capital of the Sousse Governorate. Located 140 km (87 mi) south of the capital Tunis, the city has 271,428 inhabitants (2014). Sousse is in the central-east of the country, on the Gulf of Hammamet, which is a part of the Mediterranean Sea. Its economy is based on transport equipment, processed food, olive oil, textiles, and tourism. It is home to the Université de Sousse.

Testour is a small town located in the north of Tunisia. The town is perched on the hills of Medjerda Valley, 20 km (12 mi) south-west of Medjez-el-Bab, the crossroads between Tunis, Béja, and the north of Tunisia. It was known during the Roman period, as Tichilla, which means the green grass as it is situated in the mouth of Siliana valley. It was rebuilt in the 1500s by Muslim and Jewish refugees from Andalusia who gave it a distinctive character in building and layout. Today it is a popular pilgrimage for Jews who visit Rabbi Fraji Chawat's tomb.

Thasbalta was a city and Catholic diocese in the Roman province of Africa proconsularis during the Roman Empire and of Byzacena during late antiquity. The exact location of this city is unknown but it was in the Oued es Segui river valley, north of Tozeur in the Sahel region of Tunisia.
Tozeur is an oasis and a city in south west Tunisia. The city is located north-west of Chott el Djerid, in between this Chott and the smaller Chott el Gharsa. It is the capital of the Tozeur Governorate. It was the site of the ancient city and former bishopric Tusuros, which remains a Latin Catholic titular see.

Tunis is the capital and largest city of Tunisia. The greater metropolitan area of Tunis, often referred to as "Grand Tunis", has about 2,700,000 inhabitants. As of 2020, it is the fourth-largest city in the Maghreb region and the sixteenth-largest in the Arab world.

Vassinassa was an ancient Roman–Berber city in the province of Byzacena. The exact location of the town is not known for certain, but it was in northern Tunisia.

Vegesela in Byzacena was a Roman Era town tentatively identified with ruins at Henchir-Recba in modern Tunisia. The town was in the Roman province of Byzacena.

The Diocese of Zella is a suppressed and titular see of the Roman Catholic Church.