
The Alpine Botanical Garden of Campo Imperatore is a botanical garden for alpine plants located at Campo Imperatore, L'Aquila, Abruzzo, Italy. It is operated by the University of L'Aquila.
The Arboreto Mediterraneo del Limbara is an arboretum located within the Foresta Demaniale di Monte Limbara Sud, at Via Roma, 62, Sassari, Sardinia, Italy. The arboretum's mission is to collect and preserve species native to the Mediterranean region. It contains walking paths.

The Giardino Bardini is an Italian Renaissance garden of the Villa Bardini in the hilly part of Oltrarno, offering fine views of Florence, Italy. Opened only recently to the public, it is one of Florence's well kept secrets.
The Botanical Garden of Naples, Italy is a research facility of the University of Naples Federico II.

The Chanousia Alpine Botanical Garden is an alpine botanical garden located at 2170 meters altitude near Mont Blanc, at the Little St Bernard Pass, in the Italian commune of La Thuile. It was founded by Valdostan abbot and botanist Pierre Chanoux. It is open daily in the warmer months.

The Giardini Botanici dell'Isola Madre are historic botanical gardens located on the grounds of Isola Madre in the Borromean Islands of Lake Maggiore, accessible by ferry from Stresa, Province of Verbano-Cusio-Ossola, Piedmont, Italy. They are open daily in the warmer months; an admission fee is charged.

The Giardini Botanici Villa Taranto are botanical gardens located on the western shore of Lake Maggiore in Pallanza, Province of Verbano-Cusio-Ossola, Italy. They are open daily; an admission fee is charged.

Giardino Papadopoli is a terraced garden filled with shade trees in the Venetian sestiere of Santa Croce, between the Venezia Santa Lucia train station and Piazzale Roma. Its area is about 8,800 sq. m.

Giardini Ravino is an Italian botanical garden specialized in succulent plants and cacti, located on the island of Ischia, Campania, southern Italy, in the Tyrrhenian Sea.
The Giardino Alpino "Antonio Segni" is an alpine botanical garden located at 1,714 meters altitude in Refugio Vazzoler, Gruppo del Civetta, Col Negro di Pelsa, Taibon Agordino, Province of Belluno, Veneto, Italy. It is privately owned with a municipal affiliation, and open daily when Refugio Vazzoler is open.
The Giardino Botanico Alpinia is a botanical garden specializing in alpine plants, located at 800 m altitude above Stresa on Lake Maggiore, Province of Verbano-Cusio-Ossola, Piedmont, Italy. It can be reached via the Lido di Carciano - Alpino - Mottarone cable car, and is open daily in the warmer months.

The Giardino Botanico Alpino di Passo Coe is a municipal nature preserve and alpine botanical garden located at an elevation of 1612 meters on Monte Marònia in Passo Coe, about 5 km southeast of Folgaria, Trentino, Italy. It is managed by the Museo Civico Rovereto, and open Tuesdays through Sundays in the warmer months.

The Giardino Botanico Alpino San Marco is an alpine nature preserve and botanical garden located at 1040 meters altitude, at Km 44 of SP 46, Pian delle Fugazze, Malga Prà, Valli del Pasubio, Province of Vicenza, Veneto, Italy. It is open at weekends during the warmer months.

The Giardino Botanico Alpino Valderia is a botanical garden and nature preserve located in the Parco Naturale Alpi Marittime, Corso Dante Livio Bianco 5, Valdieri, Province of Cuneo, Piedmont, Italy.

The Giardino Botanico Carsiana is a botanical garden located within the municipality of Sgonico (Zgonik) between the villages of Gabrovizza and Sgonico, Province of Trieste, Friuli-Venezia Giulia, Italy. It is open every morning except Mondays and the afternoons at the weekend and on public holidays between April and mid-October. An admission fee is charged.
The Giardino botanico Clelia Durazzo Grimaldi is a small botanical garden located on the grounds of the Villa Durazzo-Pallavicini in Pegli, a suburb of Genoa, Italy. It is a part of the romantic historical park made by Ignazio Pallavicini in 1846.

The giardino Botanico di Oropa is a nature preserve and botanical garden located at an altitude of 1200 m in Oropa about 12 km northwest of Biella, Piedmont, Italy. Situated near the Black Virgin sanctuary, it is open daily except Mondays in the warmer months.

The Giardino Botanico e Arboreto Appenninico del Parco Nazionale d'Abruzzo is a botanical garden and arboretum located in the Parco Nazionale d'Abruzzo, Lazio e Molise at via Santa Lucia, 67032 Pescasseroli, Province of L'Aquila, Abruzzo, Italy.
The Giardino Botanico Fondazione André Heller, also known as the Giardino Botanico A. Hruska, is a botanical garden located on the grounds of the André Heller Foundation above Lake Garda, in via Roma, Gardone Riviera, Province of Brescia, Lombardy, Italy. It is open daily in the warmer months.

The Botanical Garden at the Sorgenti del Cavuto is a botanical garden in Anversa degli Abruzzi, province of L'Aquila, Abruzzo, central-southern Italy. The garden was established in 1999, and is managed by the municipality of Anversa degli Abruzzi in agreement with the World Wide Fund for Nature. It contains about 380 native plant species, of which 45 are classified as endangered.

The Giardino Botanico Mediterraneo is a botanical garden located along the Adriatic Sea about 2 kilometers north of San Salvo, Province of Chieti, Abruzzo, Italy. It is open Monday through Friday in the warmer months; an admission fee is charged.

The Giardino Botanico Montano di Pratorondanino is a nature preserve and botanical garden located at 750 meters altitude in Pratorondanino, Campo Ligure, Province of Genoa, Liguria, Italy. It is open during the warmer months.

The Giardino Botanico Preistorico di Molina di Ledro is a botanical garden on the grounds of the Museo delle Palafitte del Lago di Ledro, located in Val di Ledro near Lake Garda at Via Lungolago, 1, Molina di Ledro, Trentino, Italy. It is open daily in the warmer months; an admission fee is charged.
The Giardino Botanico Tropicale dell'Istituto Agronomico per l'Oltremare is a botanical garden specializing in tropical plants. It is located at via Antonio Cocchi 4, Florence, Tuscany, Italy, and open without charge on Thursday mornings and by appointment.

The Giardino dei Semplici, also known as the Orto Botanico dell'Università D'Annunzio is a botanical garden in Chieti, Abruzzo, central Italy, operated by the D'Annunzio University. It is officially recognized as an area of regional interest.
Giardino dell'Iris is a botanical garden specializing in the cultivation of iris flowers, symbol of Florence since 1251. It is located on the corner of Viale dei Colli and Piazzale Michelangelo in Florence, Italy, and open daily without charge from May 2–20 every year.

The Giardino delle Erbe "A. Rinaldi Ceroni", formerly known as the Giardino Officinale di Casola Valsenio, is a botanical garden specializing in herbs, located Via del Corso 2/1, 48010 Casola Valsenio, Province of Ravenna, Emilia-Romagna, Italy. It is open during the months of September and October.
Giardino delle rose is a garden park in the Oltrarno district of Florence, in Tuscany, Italy. It is located below the Piazzale Michelangelo and offers a commanding view of the city.

The Giardino Montano Linasia is a nature preserve and botanical garden located at 720 meters altitude near Monte Linas in San Benedetto, Iglesias, Province of Carbonia-Iglesias, Sardinia, Italy. Opening days depend on the time of year; an admission fee is charged.
The Orto Botanico Comunale di Lucca is a botanical garden located at Via del Giardino Botanico, 14, Lucca, Italy, and operated by the city. It is open daily during the warmer months, and weekday mornings off-season. An admission fee is charged.

The Orto Botanico del Mediterraneo is a botanical garden located on the grounds of the Museo di storia naturale del Mediterraneo at Livorno, Tuscany, Italy. It contains groupings of plants typical to various locations along the Mediterranean Sea, with each group in a specific soil.
The Orto Botanico del Monte Baldo, also known as the Orto Botanico di Novezzina or Orto Botanico d'Europa, is a botanical garden located at 1232 meters altitude on Monte Baldo, Via General Graziani 10, Novezzina, Ferrara di Monte Baldo, Province of Verona, Veneto, Italy. It is open daily in the warmer months; an admission fee is charged.

The Orto Botanico dell'Università dell'Aquila, also known as the Orto Botanico di Collemaggio, is a botanical garden in L'Aquila, Abruzzo, central Italy, operated by the University of L'Aquila. Measuring 5.5 hectares, it is located near the basilica di Collemaggio.

The Orto Botanico dell'Università della Tuscia is a natural area and botanical garden operated by Tuscia University and located at Località Bulicame, Strada S. Caterina, Viterbo, Lazio, Italy.
The Orto Botanico dell'Università di Lecce, also known as the Orto Botanico di Lecce, is a botanical garden operated by the University of Lecce and located at Via Provinciale Lecce-Monteroni, 73100, Lecce, Apulia, Italy.

The Orto Botanico di Brera is a botanical garden located behind Palazzo Brera at Via Brera 28 in the center of Milan, Lombardy, Italy, and operated by the Istituto di Fisica Generale Applicata of the University of Milan. It is open weekdays without charge.
The Orto Botanico di Parma, also known as the Orto Botanico dell'Università di Parma, is a botanical garden maintained by the University of Parma. It is located on the Viale Martiri della Libertà, Parma, Italy, and open daily without charge.
The Orto botanico di Pisa, also known as the Orto Botanico dell'Università di Pisa, is a botanical garden operated by the University of Pisa, and located at via Luca Ghini 5, Pisa, Italy.
The Orto Botanico di Villa Beuca is a botanical garden situated in Beuca, on a terrace nearly 100 meters above the sea located around the Villa Beuca not far from the slopes of the Regional Natural Park of Beigua, in Cogoleto, Province of Genoa, Liguria, Italy. The garden is currently open to the public by appointment on Saturday: for groups and schools, guided tours may be organized; an admission fee is charged.
The Orto Botanico Forestale dell'Abetone is a botanical garden located in Fontana Vaccaia, Abetone Cutigliano, Province of Pistoia, Tuscany, central Italy.

The Orto Botanico "Pierina Scaramella", also known as the Orto Botanico dell'Università di Urbino, is a botanical garden maintained by the University of Urbino, and located at via Bramante, 28, Urbino, Marche, Italy. the entrance fee is 1 euro.

The Orto dei Semplici Elbano is a botanical garden located at 160 meters elevation next to the Eremo di Santa Caterina in Rio nell'Elba on the island of Elba, Province of Livorno, Italy. It is open daily except Mondays in the warmer months; an admission fee is charged.
The Giardino Alpino Paradisia or Jardin alpin Paradisia (French), is an alpine nature preserve and botanical garden located at 1700 meters altitude in the Gran Paradiso National Park at Valnontey, Cogne, Aosta Valley, Italy. It is open daily in the warmer months.

The Parco Botanico Friulano "Cormor", also known as the Parco del Cormor or Parco Botanico del Cormôr, is a municipal park and botanical garden located in Udine, Friuli, Italy.

The University of Ferrara Botanic Garden is a 4,500 square metre botanical garden operated by the University of Ferrara. It is located at Corso Porta Mare, 2b, I-44100 Ferrara, Emilia-Romagna, Italy, and is open weekday mornings. Admission is free.

The Villa L'Ambrogiana was a rural palace or villa built during the late-Renaissance by Ferdinand I de' Medici; it is located at the confluence of the rivers Pesa and Arno, in the municipality of Montelupo Fiorentino.